Bo Li: Chemiluminescent H2S and peroxynitrite probes synthesis

Hydrogen sulfide (H2S) and peroxynitrite are important biological signaling molecules that have been recognized alongside nitric oxide and carbon monoxide which could impact multiple physiological functions. To detect them, there has been a focus on developing fluorescent probes to target particular analytes; however, fluorescent probes lack sensitivity and depth penetration due to background autofluorescence and light scattering. Chemiluminescence does not require light excitation, which greatly reduces the amount of autofluorescence and photoactivation. In order to detect them in living systems with high sensitivity, a series of sterically stabilized 1,2-dioxetane chemiluminescent reduction-reaction based hydrogen sulfide probes have been synthesized.