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Daily Planet: Star Wars come to life in SMU chemist’s invention

Long ago, sort of, scenes from Star Wars triggered a child’s imagination, so that today it’s informed one of his research goals as a chemist.

Discover Canada’s science magazine show Daily Planet reported on the research of SMU organic chemist Alex Lippert, an assistant professor in the Department of Chemistry in SMU’s Dedman College of Humanities and Sciences.

Lippert’s team develops synthetic organic compounds that glow in reaction to certain conditions. He led his lab in developing a new technology that uses photoswitch molecules to craft 3-D light structures — not holograms — that are viewable from 360 degrees. An economical method for shaping light into an infinite number of volumetric objects, the technology will be useful in a variety of fields, from biomedical imaging, education and engineering, to TV, movies, video games and more.

For biomedical imaging, Lippert says the nearest-term application of the technique might be in high-volume pre-clinical animal imaging, but eventually the technique could be applied to provide low-cost internal imaging in the developing world, or less costly imaging in the developed world.

The Daily Planet segment aired Dec. 12, 2017.

Lippert’s lab includes four doctoral students and five undergraduates who assist in his research. He recently received a prestigious National Science Foundation Career Award, expected to total $611,000 over five years, to fund his research into alternative internal imaging techniques.

NSF Career Awards are given to tenure-track faculty members who exemplify the role of teacher-scholars through outstanding research, excellent education and the integration of education and research in American colleges and universities.

Lippert joined SMU in 2012. He was previously a postdoctoral researcher at the University of California, Berkeley, and earned his Ph.D. at the University of Pennsylvania, and Bachelor of Science at the California Institute of Technology.

Watch the full Dec. 12 show.

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Cosmos: Painting with light in three dimensions

A new technique uses photoswitch molecules to create three-dimensional images from pure light.

Australia’s quarterly science magazine Cosmos covered the research of SMU organic chemist Alex Lippert, an assistant professor in the Department of Chemistry in SMU’s Dedman College of Humanities and Sciences.

Lippert’s team develops synthetic organic compounds that glow in reaction to certain conditions. He led his lab in developing a new technology that uses photoswitch molecules to craft 3-D light structures — not holograms — that are viewable from 360 degrees. The economical method for shaping light into an infinite number of volumetric objects would be useful in a variety of fields, from biomedical imaging, education and engineering, to TV, movies, video games and more.

For biomedical imaging, Lippert says the nearest-term application of the technique might be in high-volume pre-clinical animal imaging, but eventually the technique could be applied to provide low-cost internal imaging in the developing world, or less costly imaging in the developed world.

Cosmos reporter Joel F. Hooper wrote about the new technology in “Painting with light in three dimensions,” which published online July 14, 2017.

Lippert’s lab includes four doctoral students and five undergraduates who assist in his research. He recently received a prestigious National Science Foundation Career Award, expected to total $611,000 over five years, to fund his research into alternative internal imaging techniques.

NSF Career Awards are given to tenure-track faculty members who exemplify the role of teacher-scholars through outstanding research, excellent education and the integration of education and research in American colleges and universities.

Lippert joined SMU in 2012. He was previously a postdoctoral researcher at the University of California, Berkeley, and earned his Ph.D. at the University of Pennsylvania, and Bachelor of Science at the California Institute of Technology.

Read the full story.

EXCERPT:

By Joel F. Hooper
Cosmos

Those of us who grew up watching science fiction movies and TV shows imagined our futures to be filled with marvellous gadgets, but we’ve sometimes been disappointed when science fails to deliver. We can’t take a weekend trip to Mars yet, and we’re still waiting for hoverboards that actually hover.

But in the case of 3-D image projection, the technology used by R2D2 in Star Wars is making its way into reality. Using advances in fluorescent molecules that can be switched on by UV light, scientists at Southern Methodist University in Dallas have created a method for producing images and animations by structuring light in 3-dimentions.

The technology uses a solution of fluorescent molecules called rhodamines, which have the potential to emit visible light when they are excited by a light beam of the right wavelength. But these molecules are usually in an inactive state, and must be “switched on” by UV light before they can become emitters. When a UV light or visible light beam alone shines through the solution, the rhodamines to not emit light. But where these two beams intersect, the emitting molecules are both switched on and excited, and can produce a small glowing 3D pixel, known as a voxel.

When a number of voxels are produced at once, using two projectors positioned at 90° to a flask containing a solution of the fluorescent molecules, a 3D image is produced.

“Our idea was to use chemistry and special photoswitch molecules to make a 3D display that delivers a 360-degree view,” says Alexander Lippert, lead author of the study. “It’s not a hologram, it’s really three-dimensionally structured light.”

Read the full story.

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Better than Star Wars: Chemistry discovery yields 3-D table-top objects crafted from light

Photoswitch chemistry allows construction of light shapes into structures that have volume and are viewable from 360 degrees, making them useful for biomedical imaging, teaching, engineering, TV, movies, video games and more

A scientist’s dream of 3-D projections like those he saw years ago in a Star Wars movie has led to new technology for making animated 3-D table-top objects by structuring light.

The new technology uses photoswitch molecules to bring to life 3-D light structures that are viewable from 360 degrees, says chemist Alexander Lippert, Southern Methodist University, Dallas, who led the research.

The economical method for shaping light into an infinite number of volumetric objects would be useful in a variety of fields, from biomedical imaging, education and engineering, to TV, movies, video games and more.

“Our idea was to use chemistry and special photoswitch molecules to make a 3-D display that delivers a 360-degree view,” said Lippert, an assistant professor in the SMU Department of Chemistry. “It’s not a hologram, it’s really three-dimensionally structured light.”

Key to the technology is a molecule that switches between non-fluorescent and fluorescent in reaction to the presence or absence of ultraviolet light.

The new technology is not a hologram, and differs from 3-D movies or 3-D computer design. Those are flat displays that use binocular disparity or linear perspective to make objects appear three-dimensional when in fact they only have height and width and lack a true volume profile.

“When you see a 3-D movie, for example, it’s tricking your brain to see 3-D by presenting two different images to each eye,” Lippert said. “Our display is not tricking your brain — we’ve used chemistry to structure light in three actual dimensions, so no tricks, just a real three-dimensional light structure. We call it a 3-D digital light photoactivatable dye display, or 3-D Light Pad for short, and it’s much more like what we see in real life.”

At the heart of the SMU 3-D Light Pad technology is a “photoswitch” molecule, which can switch from colorless to fluorescent when shined with a beam of ultraviolet light.

The researchers discovered a chemical innovation for tuning the photoswitch molecule’s rate of thermal fading — its on-off switch — by adding to it the chemical amine base triethylamine.

Now the sky is the limit for the new SMU 3-D Light Pad technology, given the many possible uses, said Lippert, an expert in fluorescence and chemiluminescence — using chemistry to explore the interaction between light and matter.

For example, conference calls could feel more like face-to-face meetings with volumetric 3-D images projected onto chairs. Construction and manufacturing projects could benefit from rendering them first in 3-D to observe and discuss real-time spatial information. For the military, uses could include tactical 3-D replications of battlefields on land, in the air, under water or even in space.

Volumetric 3-D could also benefit the medical field.

“With real 3-D results of an MRI, radiologists could more readily recognize abnormalities such as cancer,” Lippert said. “I think it would have a significant impact on human health because an actual 3-D image can deliver more information.”

Unlike 3-D printing, volumetric 3-D structured light is easily animated and altered to accommodate a change in design. Also, multiple people can simultaneously view various sides of volumetric display, conceivably making amusement parks, advertising, 3-D movies and 3-D games more lifelike, visually compelling and entertaining.

Lippert and his team in The Lippert Research Group report on the new technology and the discovery that made it possible in the article “A volumetric three-dimensional digital light photoactivatable dye display,” published in the journal Nature Communications.

Some of the 3-D images generated with the new technology are viewable in this video.

Co-authors are Shreya K. Patel, lead author, and Jian Cao, both students in the SMU Department of Chemistry.

Genesis of an idea — cinematic inspiration
The idea to shape light into volumetric animated 3-D objects came from Lippert’s childhood fascination with the movie “Star Wars.” Specifically he was inspired when R2-D2 projects a hologram of Princess Leia. Lippert’s interest continued with the holodeck in “Star Trek: The Next Generation.”

“As a kid I kept trying to think of a way to invent this,” Lippert said. “Then once I got a background in chemistry molecules that interact with light, and an understanding of photoswitches, it finally dawned on me that I could take two beams of light and use chemistry to manipulate the emission of light.”

Key to the new technology was discovering how to turn the chemical photoswitch off and on instantly, and generating light emissions from the intersection of two different light beams in a solution of the photoactivatable dye, he said.

SMU graduate student in chemistry Jian Cao hypothesized the activated photoswitch would turn off quickly by adding the base. He was right.

“The chemical innovation was our discovery that by adding one drop of triethylamine, we could tune the rate of thermal fading so that it instantly goes from a pink solution to a clear solution,” Lippert said. “Without a base, the activation with UV light takes minutes to hours to fade back and turn off, which is a problem if you’re trying to make an image. We wanted the rate of reaction with UV light to be very fast, making it switch on. We also wanted the off-rate to be very fast so the image doesn’t bleed.”

SMU 3-D Light Pad
In choosing among various photoswitch dyes, the researchers settled on N-phenyl spirolactam rhodamines. That particular class of rhodamine dyes was first described in the late 1970s and made use of by Stanford University’s Nobel prize-winning W.E. Moerner.

The dye absorbs light within the visible region, making it appropriate to fluoresce light. Shining it with UV radiation, specifically, triggers a photochemical reaction and forces it to open up and become fluorescent.

Turning off the UV light beam shuts down fluorescence, diminishes light scattering, and makes the reaction reversible — ideal for creating an animated 3-D image that turns on and off.

“Adding triethylamine to switch it off and on quickly was a key chemical discovery that we made,” Lippert said.

To produce a viewable image they still needed a setup to structure the light.

Structuring light in a table-top display
The researchers started with a custom-built, table-top, quartz glass imaging chamber 50 millimeters by 50 millimeters by 50 millimeters to house the photoswitch and to capture light.

Inside they deployed a liquid solvent, dichloromethane, as the matrix in which to dissolve the N-phenyl spirolactam rhodamine, the solid, white crystalline photoswitch dye.

Next they projected patterns into the chamber to structure light in two dimensions. They used an off-the-shelf Digital Light Processing (DLP) projector purchased at Best Buy for beaming visible light.

The DLP projector, which reflects visible light via an array of microscopically tiny mirrors on a semiconductor chip, projected a beam of green light in the shape of a square. For UV light, the researchers shined a series of UV light bars from a specially made 385-nanometer Light-Emitting Diode projector from the opposite side.

Where the light intersected and mixed in the chamber, there was displayed a pattern of two-dimensional squares stacked across the chamber. Optimized filter sets eliminated blue background light and allowed only red light to pass.

To get a static 3-D image, they patterned the light in both directions, with a triangle from the UV and a green triangle from the visible, yielding a pyramid at the intersection, Lippert said.

From there, one of the first animated 3-D images the researchers created was the SMU mascot, Peruna, a racing mustang.

“For Peruna — real-time 3-D animation — SMU undergraduate student Shreya Patel found a way to beam a UV light bar and keep it steady, then project with the green light a movie of the mustang running,” Lippert said.

So long Renaissance
Today’s 3-D images date to the Italian Renaissance and its leading architect and engineer.

“Brunelleschi during his work on the Baptistery of St. John was the first to use the mathematical representation of linear perspective that we now call 3-D. This is how artists used visual tricks to make a 2-D picture look 3-D,” Lippert said. “Parallel lines converge at a vanishing point and give a strong sense of 3-D. It’s a useful trick but it’s striking we’re still using a 500-year-old technique to display 3-D information.”

The SMU 3-D Light Pad technology, patented in 2016, has a number of advantages over contemporary attempts by others to create a volumetric display but that haven’t emerged as commercially viable.

Some of those have been bulky or difficult to align, while others use expensive rare earth metals, or rely on high-powered lasers that are both expensive and somewhat dangerous.

The SMU 3-D Light Pad uses lower light powers, which are not only cheaper but safer. The matrix for the display is also economical, and there are no moving parts to fabricate, maintain or break down.

Lippert and his team fabricated the SMU 3-D Light Pad for under $5,000 through a grant from the SMU University Research Council.

“For a really modest investment we’ve done something that can compete with more expensive $100,000 systems,” Lippert said. “We think we can optimize this and get it down to a couple thousand dollars or even lower.”

Next Gen: SMU 3-D Light Pad 2.0
The resolution quality of a 2-D digital photograph is stated in pixels. The more pixels, the sharper and higher-quality the image. Similarly, 3-D objects are measured in voxels — a pixel but with volume. The current 3-D Light Pad can generate more than 183,000 voxels, and simply scaling the volume size should increase the number of voxels into the millions – equal to the number of mirrors in the DLP micromirror arrays.

For their display, the SMU researchers wanted the highest resolution possible, measured in terms of the minimum spacing between any two of the bars. They achieved 200 microns, which compares favorably to 100 microns for a standard TV display or 200 microns for a projector.

The goal now is to move away from a liquid vat of solvent for the display to a solid cube table display. Optical polymer, for example, would weigh about the same as a TV set. Lippert also toys with the idea of an aerosol display.

The researchers hope to expand from a monochrome red image to true color, based on mixing red, green and blue light. They are working to optimize the optics, graphics engine, lenses, projector technology and photoswitch molecules.

“I think it’s a very fascinating area. Everything we see — all the color we see — arises from the interaction of light with matter,” Lippert said. “The molecules in an object are absorbing a wavelength of light and we see all the rest that’s reflected. So when we see blue, it’s because the object is absorbing all the red light. What’s more, it is actually photoswitch molecules in our eyes that start the process of translating different wavelengths of light into the conscious experience of color. That’s the fundamental chemistry and it builds our entire visual world. Being immersed in chemistry every day — that’s the filter I’m seeing everything through.”

The SMU discovery and new technology, Lippert said, speak to the power of encouraging young children.

“They’re not going to solve all the world’s problems when they’re seven years old,” he said. “But ideas get seeded and if they get nurtured as children grow up they can achieve things we never thought possible.” — Margaret Allen, SMU

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SMU chemist wins prestigious NSF Career Award

Alex Lippert’s research uses chemistry to develop affordable, glowing internal imaging techniques

SMU chemist Alex Lippert has received a prestigious National Science Foundation Career Award, expected to total $611,000 over five years, to fund his research into alternative internal imaging techniques.

NSF Career Awards are given to tenure-track faculty members who exemplify the role of teacher-scholars through outstanding research, excellent education and the integration of education and research in American colleges and universities.

Lippert, an assistant professor in the Department of Chemistry in SMU’s Dedman College of Humanities and Sciences, is an organic chemist and adviser to four doctoral students and five undergraduates who assist in his research.

Lippert’s team develops synthetic organic compounds that glow in reaction to certain conditions. For example, when injected into a mouse’s tumor, the compounds luminesce in response to the cancer’s pH and oxygen levels. Place that mouse in a sealed dark box with a sensitive CCD camera that can detect low levels of light, and images can be captured of the light emanating from the mouse’s tumor.

“We are developing chemiluminescent imaging agents, which basically amounts to a specialized type of glow-stick chemistry,” Lippert says. “We can use this method to image the insides of animals, kind of like an MRI, but much cheaper and easier to do.”

Lippert says the nearest-term application of the technique might be in high-volume pre-clinical animal imaging, but eventually the technique could be applied to provide low-cost internal imaging in the developing world, or less costly imaging in the developed world.

But first, there are still a few ways the technique can be improved, and that’s where Lippert says the grant will come in handy.

“In preliminary studies, we needed to directly inject the compound into the tumor to see the chemistry in the tumor,” Lippert says. “One thing that’s funded by this grant is intravenous injection capability, where you inject a test subject and let the agent distribute through the body, then activate it in the tumor to see it light up.”

Another challenge the team will use the grant to explore is making a compound that varies by color instead of glow intensity when reacting to cancer cells. This will make it easier to read images, which can sometimes be buried under several layers of tissue, making the intensity of the glow difficult to interpret.

“We’re applying the method to tumors now, but you could use similar designs for other types of tissues,” Lippert says. “The current compound reacts to oxygen levels and pH, which are important in cancer biology, but also present in other types of biology, so it can be more wide-ranging than just looking at cancer.”

“This grant is really critical to our ability to continue the research going forward,” Lippert adds. “This will support the reagents and supplies, student stipends, and strengthen our collaboration with UT Southwestern Medical Center. Having that funding secure for five years is really nice because we can now focus our attention on the actual science instead of writing grants. It’s a huge step forward in our research progress.”

Lippert joined SMU in 2012. He was a postdoctoral researcher at the University of California, Berkeley, from 2009-12, earned his Ph.D. at the University of Pennsylvania in 2008 and earned a Bachelor of Science at the California Institute of Technology in 2003.

The National Science Foundation (NSF) is an independent federal agency created by Congress in 1950 “to promote the progress of science; to advance the national health, prosperity, and welfare; to secure the national defense…” NSF is the funding source for approximately 24 percent of all federally supported basic research conducted by America’s colleges and universities. — Kenny Ryan, SMU

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Study solves mystery of how plants use sunlight to tell time via cell protein signaling

Discovery may someday allow farmers to grow crops in climates where they currently won’t grow and allows scientists to make a subtle, targeted mutation to a specific native plant protein

Findings of a new study solve a key mystery about the chemistry of how plants tell time so they can flower and metabolize nutrients.

The process — a subtle chemical event — takes place in the cells of every plant every second of every day.

The new understanding means farmers may someday grow crops under conditions or in climates where they currently can’t grow, said chemist Brian D. Zoltowski, Southern Methodist University, Dallas, who led the study.

“We now understand the chemistry allowing plants to maintain a natural 24-hour rhythm in sync with their environment. This allows us to tune the chemistry, like turning a dimmer switch up or down, to alter the organism’s ability to keep time,” Zoltowski said. “So we can either make the plant’s clock run faster, or make it run slower. By altering these subtle chemical events we might be able to rationally redesign a plant’s photochemistry to allow it to adapt to a new climate.”

Specifically, the researchers figured out the chemical nuts and bolts of how a chemical bond in the protein Zeitlupe forms and breaks in reaction to sunlight, and the rate at which it does so, to understand how proteins in a plant’s cells signal the plant when to bloom, metabolize, store energy and perform other functions.

Zoltowski’s team, with collaborators at the University of Washington and Ohio State University, have made plant strains with specific changes to the way they are able to respond to blue-light.

“With these plants we demonstrate that indeed we can tune how the organisms respond to their environment in an intelligible manner,” Zoltowski said.

Zoltowski and his colleagues made the discovery by mapping the crystal structure of a plant protein whose function is to measure the intensity of sunlight. The protein is able to translate light intensity to a bond formation event that allows the plant to track the time of day and tell the plant when to bloom or metabolize nutrients.

A plant uses visual cues to constantly read every aspect of its environment and retune its physiological functions to adapt accordingly. Some of these cues are monitored by plant proteins that absorb and transmit light signals — called photoreceptors. The research team specifically studied two key photoreceptors, Zeitlupe (Zite-LOO-puh) and FKF-1.

“Plants have a very complex array of photoreceptors absorbing all different wavelengths of light to recognize every aspect of their environment and adapt accordingly,” said Zoltowski, an assistant professor in the SMU Department of Chemistry. “All their cells and tissue types are working in concert with each other.”

The finding was reported in the article “Kinetics of the LOV domain of Zeitlupe determine its circadian function in Arabidopsis” in the journal eLIFE online in advance of print publication.

Co-author and lead author is Ashutosh Pudasaini, a doctoral graduate from the SMU Department of Chemistry who is now a postdoctoral fellow at the University of Texas Southwestern Medical School, Dallas. Other co-authors are Jae Sung Shim, Young Hun Song and Takato Imaizumi, University of Washington, Seattle; Hua Shi and David E. Somers, Ohio State University; and Takatoshi Kiba, RIKEN Center for Sustainable Resource Science, Japan.

The research is funded through a grant from the National Institute of General Medical Sciences of the National Institutes of Health awarded to Zoltowski’s lab.

Nighttime is the right time for plants to grow
“If you live in the Midwest, people say you hear the corn growing at night,” said Zoltowski, who grew up in rural Wisconsin.

“During the day, a plant is storing as much energy as it can by absorbing photons of sunlight, so that during the evening it can do all its metabolism and growth and development. So there’s this separation between day and night.”

Plants measure these day and night oscillations as well as seasonal changes. Knowledge already existed of the initial chemistry, biology and physiology of that process.

In addition, Zoltowski and colleagues published in 2013 the discovery that the amino acids in Zeitlupe — working like a dimmer switch — gradually get more active as daytime turns to evening, thereby managing the 24-hour Circadian rhythm. Additionally, they found that FKF-1 is very different from Zeitlupe. FKF-1 switches on with morning light and measures seasonal changes, otherwise called photoperiodism.

But a knowledge gap remained. It was a mystery how the information is integrated by the organism.

“Ultimately that has to be related to some kind of chemical event occurring, some kind of chemical timekeeper,” Zoltowski said. “So by following that trail we figured out how the chemistry works.”

Dark state and light state snapshots
The problem required a two-pronged approach: Solving the structure of the protein to understand how forming and breaking bonds changes how the organism perceives its environment; and solving the chemistry, specifically the crystal structures of the protein’s dark and light states.

That process yielded a snapshot of the protein in the dark state and a snapshot of the protein in the light state, so the researchers could watch changes in protein structure in response to the bond-forming event.

From there, the researchers made mathematical models 1) that explain how the chemistry of the bond breaking and bond forming event, and the rate at which it occurs, should affect the organism; and 2) that design mutations to the protein that affect how it goes from the dark state to the light state to block that process.

Standard techniques yielded the discovery
The team used a few standard techniques. To get at the chemistry, they deployed ultra-violet visible spectroscopy to measure how efficiently proteins absorb light. They followed differences in the absorption spectrum, seeing what wavelengths are absorbed, to track chemical changes between the dark and the light states.

On the structure side, they crystallized the proteins and collected data at synchrotron sources at Cornell University, then mapped out like a puzzle where all the electrons are located in the crystal. From there they could fit and build — amino acid by amino acid — the protein, yielding a three-dimensional image of where every atom in the protein is located.

“This gives us pictures and snapshots of all those discrete events, where then we can look at how the atoms are moving and changing from one to the other,” Zoltowski said. “That allows us to see the bonds forming, the bonds breaking, and how the rest of the protein changes in response to that.”

Why didn’t we think of that?
The question has been an important one in the field, but challenging technical hurdles thwarted solutions, said Zoltowski. The key for his team was persistence and years of experience.

“This is not an easy protein to work with — it’s difficult to get crystals of these proteins. It requires a protein that is stable enough and will interact in a way that it yields a perfectly ordered crystal. So it’s difficult to do the chemistry and the structures. Researchers have struggled with getting adequate amounts of protein to be able to do these types of characterizations,” he said.

Think of it like a diamond, Zoltowski said, which is a perfectly ordered crystal that is just carbon atoms arranged in a specific way.

“Zeitlupe and FKF-1 have thousands of atoms in each protein, and in order to get a crystal, each molecule of the protein needs to arrange itself with the same type of accuracy and precision as carbon atoms in a diamond. Getting that to occur, where they pack nicely together, is non trivial. And some proteins just are really challenging to work with.”

Zoltowski and his colleagues have been fortunate in having years of experience working with these families of proteins, called the Light-oxygen-voltage-sensing domains, or LOV domains, for short.

“So we’ve developed a lot of skills and techniques over the years that can get over some of the technical hurdles,” he said. “So just from gaining experience over time, we’ve gotten better with working with some very difficult proteins. It makes something that is challenging, much more tractable for our lab.”

Does this apply to all LOV proteins in every plant?
Zeitlupe is a German word that means slow motion. The protein was dubbed Zeitlupe because scientists discovered when they found mutations of this protein previously that it made the Circadian clock run slower. It naturally altered the way the organism perceived time.

“We wanted to understand the proteins well enough that we could selectively alter the chemistry, or selectively alter the structure, to create mutations that would be testable in the organism,” Zoltowski said. “We wanted a predictive model that would tell us that these mutations that affect the kinetics — the rate at which this bond breaks — should do ‘X’ in the organism.”

The team’s new discovery results in hybrid plants — something nature already does and has done for millions of years through the process of evolution so that plants adapt to survive.

“We’re not putting anything into the plant or changing its genetics,” Zoltowski said. “We’re making a very subtle, targeted mutation to a specific protein that already is a native plant protein — and one that we’ve shown in this paper has evolved considerably throughout various different agricultural crops to already do this.”

The discovery gives scientists the ability to rationally interpret environmental information affecting a plant in order to introduce mutations, instead of relying on selective breeding to achieve a targeted mutation to generate phenotypes that potentially allow the plant to grow in a different environment.

What’s next?
The research opens a lot of new doors, including new questions about how these proteins are changing their configuration and how other variables, like oxidative stress, couple with the plant’s global sensory networks to also alter proteins and send multiple signals from the environment.

“What we’ve learned is that you need to pay careful attention to specific parts of the protein because they’re modulating activity selectively in different categories of this family,” Zoltowski said. “If we look at the whole family of these proteins, there are key amino acids that are evolutionarily selected, so they evolve specific modulations of this activity for their own independent niche in the environment. One of the take-homes is there are areas in the protein we need to look at to see how the amino acids are now different.”

Besides the NIH grant, the lab operates with $250,000 from the American Chemical Society’s Herman Frasch Foundation for Chemical Research Grants in Agricultural Chemistry. — Margaret Allen